GIFSQ — Global Institute for Food Safety & Quality 1. Principle Whole dates that will not be cleaned or sorted again before use or sale are sampled and examined for insect infestation, mites, mould, hairs, fibres, sand, soil and other extraneous material — and, in pitted dates, for pits and pieces of pit — to […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle Green and roasted whole coffee beans are examined for insect infestation, mould, hairs, fibres, sand, soil and other extraneous material. Each analytical unit is examined bean by bean under the microscope, every bean is classified into a defect category, and the percent defectives are […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle Cocoa beans are sampled and examined for insect infestation and mould to determine compliance with the requirements of Sections 4, 5 and 7 of the Food and Drugs Act. The cut surfaces of the beans are examined visually for insects, mould and their damage, […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle Extraneous material in alimentary paste is divided into light filth and heavy filth. Light filth elements (insects, insect fragments, mites, hairs, feather barbules) are isolated by acid digestion of the product, sieving, and separation in a mineral-oil interface in a percolator. Heavy filth particles […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is applicable to the rapid detection of Listeria spp. to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. This method has been validated for a variety of environmental surfaces. The […]

GIFSQ — Global Institute for Food Safety & Quality Principle The VIDAS UP E. coli O157 (including H7) method — also known as the E. coli Phage Technology (ECPT) method — is an automated assay built on the ELFA technique (Enzyme-Linked Fluorescent Assay), for use on the VIDAS family of instruments. A Solid Phase Receptacle […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is for the rapid identification of Listeria monocytogenes to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act. It has been validated for use on isolates from methods MFLP-74, MFHPB-07, and MFHPB-30. The AccuProbe Listeria monocytogenes […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method detects enterohemorrhagic Escherichia coli O157 (EHEC) in food products and food ingredients to determine compliance with Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. It is validated for use in raw processed and raw unprocessed meats […]

GIFSQ — Global Institute for Food Safety & Quality Principle Following the detection procedure, presumptive positive colonies are subjected to a polymerase chain reaction (PCR) procedure which amplifies a specific DNA sequence of the toxin gene. The primers used in the PCR are highly specific for verotoxigenic Escherichia coli (VTEC), and do not amplify DNA […]

GIFSQ — Global Institute for Food Safety & Quality Principle Application. This method enumerates coliform bacteria in foods and environmental samples. Tolerance guidelines are specific to the product tested. This revised method replaces MFLP-85, dated October 1996. Petrifilm plates are a ready-to-use product of the 3M Company. Culture medium is coated onto films, so traditional […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method concentrates Salmonella spp. by immunomagnetic separation (IMS). It is applicable to the concentration of Salmonella spp. and has been validated for animal feed. This edition replaces MFLP-84, dated October 2001. The Dynabeads ImmunoMagenetic Separation technology uses magnetically charged polystyrene beads with Salmonella-specific polyclonal […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method detects Escherichia coli O157 in food products, to check compliance with Sections 4 and 7 of the Food and Drugs Act. A positive result must be confirmed with a cultural method. The method has been validated for the food category Raw Meat Products […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is applicable to the rapid detection of Listeria spp. in environmental surface samples, to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. It has been validated for a variety of […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is applicable to the rapid identification of Listeria monocytogenes colonies to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act. It has been validated for use on isolates from foods, as well as environmental and clinical […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is applicable to the rapid detection of Listeria monocytogenes and other Listeria spp. to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act. The method has been validated for use in raw meats, dairy products, fruits […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method detects Escherichia coli O157:H7/NM (including non-motile strains that still carry the H7 genes) in raw ground meat and raw meat trim that do not contain spices, to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act […]

GIFSQ — Global Institute for Food Safety & Quality (Health Canada, Compendium of Analytical Methods, Volume 3 — Laboratory Procedure MFLP-75, March 2016) Principle This procedure detects motile Salmonella spp. to determine compliance with Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. It is validated for raw unprocessed […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is Health Canada’s reference method for the enumeration of viable Listeria monocytogenes, applicable to all foods, for determining compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and other relevant federal regulations. A portion of the product […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method gives a rapid detection of Listeria spp. to show compliance with Sections 4 and 7 of the Food and Drugs Act. It has been validated for raw meat, raw unprocessed seafood, raw frozen vegetables, cheese made from unpasteurized milk, and environmental samples. This […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method identifies seven O serogroups of verotoxigenic Escherichia coli (VTEC) as viable colonies isolated from foods on selective plating media. It is used to determine compliance with Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. A multiplex […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method measures the water activity (a_w) of foods and food ingredients to determine compliance with Sections 4 and 7 of the Food and Drugs Act and any other relevant federal regulations. It may not be applicable to foods containing volatile components. (This revised method […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is applicable to the rapid detection of generic Escherichia coli and other coliform bacteria, to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. The method has been validated for the […]

GIFSQ — Global Institute for Food Safety & Quality Health Canada, Compendium of Analytical Methods, Volume 3 — Laboratory Procedure MFLP-63, January 2006 (replaces MFLP-63, dated April 1999) Principle This method is used to determine the water activity (aw) of foods, to establish compliance with the requirements of Sections 4 and 7 of the Food […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method determines the presence of the verotoxin genes in verotoxigenic Escherichia coli (VTEC) colonies isolated from foods. It can be used to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. Following […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is applicable to the rapid detection of Listeria spp. to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. It has been validated for use in raw and raw processed Fruit […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is applicable to the rapid detection of Listeria monocytogenes to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. It has been validated for use in ready-to-eat meat and poultry, fruit […]

GIFSQ — Global Institute for Food Safety & Quality Principle The cloth-based hybridization array system (CHAS) is a DNA amplification and hybridization platform that lets colonies isolated on plating media be checked for marker gene sequences known to be associated with specific pathogenic bacteria. The L. monocytogenes CHAS (Lm-CHAS) identifies definitive gene sequences associated with […]

GIFSQ — Global Institute for Food Safety & Quality Principle The method is applicable to the recovery and identification of priority verotoxigenic Escherichia coli (VTEC) — that is, VTEC having a high rate of association with food-borne illness. It can be used to determine compliance with Sections 4 and 7 of the Food and Drugs […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is applicable to the rapid detection of Listeria species to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. Positive results must be confirmed with a cultural method. This method has […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method detects Clostridium botulinum in honey and syrups. Botulinal spores are removed from the liquid portion of the sample by membrane filtration, the membrane is cultivated in a liquid medium, the culture is analysed for toxin, and the toxin is identified with specific botulinal […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is applicable to the rapid detection of Salmonella spp. in food products to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. Positive results must be confirmed with a cultural method. […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is applicable to the isolation and identification of Yersinia enterocolitica from foods and environmental samples to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act. This method replaces MFLP-48, dated January 1999. Y. enterocolitica is a […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method isolates the thermotolerant and microaerophilic bacteria of the genus Campylobacter from raw poultry products, raw meat unprocessed, dairy products (with the exception of cheeses), molluscan shellfish, and environmental samples (stainless steel) to determine compliance with the requirements of Sections 4 and 7 of […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method can be used to determine the chloride content of most foods. The kit gives results as % sodium chloride or ppm Cl⁻ ions on a wet weight basis. To determine % brine, the moisture content of the sample must also be determined. Where […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is applicable to the enumeration of viable sporeforming microorganisms to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act. This revised method replaces MFLP-44 dated April 1998. The aerobic sporeformers count (ASF) and the anaerobic sporeformers […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is applicable to the enumeration of viable Enterobacteriaceae in accordance with the requirements of Sections 4 and 7 of the Food and Drugs Act. This revised method replaces MFLP-43, dated May 1988. This method has been shown to produce satisfactory results with naturally-contaminated […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is applicable to the isolation, identification and enumeration of the Bacillus cereus group (with limitations as described in the method) in foods, in accordance with the requirements of Sections 4 and 7 of the Food and Drugs Act. This revised method replaces MFLP-42, […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method applies to the rapid detection of Salmonella spp. to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. It has been validated for all foods and a variety of environmental surfaces. […]

GIFSQ — Global Institute for Food Safety & Quality Principle This document gives the laboratory formulations of the agars, broths and other reagents in the Media Glossary that fall under the letters Q–R. For each medium it lists the basal medium ingredients with their quantities, any supplements, and the complete-medium preparation steps, so that the […]

GIFSQ — Global Institute for Food Safety & Quality Principle This document gives the laboratory formulations of the agars, broths and other reagents in the Media Glossary that begin with the letter N. For each medium or reagent it lists the basal medium ingredients with their quantities and the complete-medium preparation steps, so that each […]

GIFSQ — Global Institute for Food Safety & Quality Principle This document gives the laboratory formulations of the agars, broths and other reagents in the Media Glossary that begin with the letter O. For each medium it lists the basal medium ingredients with their quantities, any supplement, and the complete-medium preparation steps, so that the […]

GIFSQ — Global Institute for Food Safety & Quality Principle This procedure applies to sampling the environment for microorganisms in food establishments in support of compliance activities relative to Section 7 of the Food and Drugs Act, and Health Canada policies such as the Policy on Listeria monocytogenes in ready-to-eat (RTE) food and Canadian Food […]

GIFSQ — Global Institute for Food Safety & Quality Principle The VIDAS Easy Salmonella (SLM) method is an enzyme immunoassay run on the VIDAS instrument for the detection of Salmonella antigens, using the ELFA technique (Enzyme Linked Fluorescent Assay). The internal surface of the Solid Phase Receptacle (SPR), a pipette-tip-like disposable device, is pre-coated during […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is applicable to the rapid detection of Listeria spp. to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. It has been validated for a variety of environmental surfaces and for […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method detects, isolates, and enumerates Vibrio parahaemolyticus and/or Vibrio vulnificus in fish, including shellfish and crustaceans, to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. This revised method replaces MFLP-37, dated […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method detects Salmonella spp. to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. It has been validated for use in all foods and environmental surface samples except for two food categories: […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is applicable to the rapid detection of Listeria spp. to determine compliance with the requirements of Section 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. It has been validated for raw meat, raw fish and crustaceans, raw […]

GIFSQ — Global Institute for Food Safety & Quality Principle The VIDAS LMO 2 assay is an enzyme-linked fluorescent immunoassay (ELFA) run on the VIDAS system for the qualitative detection of Listeria monocytogenes. During production, the inner surface of the Solid Phase Receptacle (SPR) — a disposable, pipette-tip-like device — is pre-coated with anti-L. monocytogenes […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method detects Salmonella species to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. Positive results must be confirmed with a cultural method. The method has been validated for heat processed dairy […]

GIFSQ — Global Institute for Food Safety & Quality Principle Following an initial 24 hour pre-enrichment that is common with the cultural method for the isolation of Listeria monocytogenes (MFHPB-30), an aliquot of the broth is transferred onto a Palcam agar slant for an additional 18 to 24 hours. The cell suspension obtained by washing […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method detects Escherichia coli O157:H7 in select foods to determine compliance with Sections 4 and 7 of the Food and Drugs Act and other relevant federal regulations. It has been validated for dairy products, raw meat products, ready-to-eat meat and poultry products, fruit- and […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method detects Salmonella species in foods and environmental surface samples to assess compliance with Sections 4 and 7 of the Food and Drugs Act and other relevant federal regulations. Positive results must be confirmed with a cultural method. The method has been validated for […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method detects Listeria monocytogenes to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and other relevant federal regulations. It is validated for all foods and a variety of environmental surfaces on the BAX system Q7 or […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is applicable to the detection of Enterobacter sakazakii in selected foods, including powdered infant formula, dry dairy and soy ingredients, and food production environmental samples after enrichment. The BAX system is a yes/no screening tool that uses Polymerase Chain Reaction (PCR) technology for […]

GIFSQ — Global Institute for Food Safety & Quality Principle Following enrichment of samples in Shigella broth with novobiocin, a portion of the enrichment broth is treated and then subjected to a polymerase chain reaction (PCR) procedure which amplifies a 629-base-pair (bp) fragment within the 220-kbp invasive plasmids and chromosomal DNA of Shigella spp. The […]

GIFSQ — Global Institute for Food Safety & Quality Principle A portion of the food sample is enriched in a selective broth containing novobiocin, then plated onto selective-differential agars for the isolation of Shigella spp. Presumptive Shigella isolates are screened and identified by biochemical testing and serology. The method is based on the FDA Bacteriological […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method identifies strains of Vibrio parahaemolyticus by a multiplex polymerase chain reaction (PCR) that amplifies three DNA sequences at once: the r72h fragment of the V. parahaemolyticus genome (the species identifier), the tdh gene (thermostable direct hemolysin), and the trh gene (tdh-related hemolysin). The […]

GIFSQ — Global Institute for Food Safety & Quality Principle The cloth-based hybridization array system (CHAS) is a DNA amplification and hybridization platform that allows colonies isolated on plating media to be checked for marker gene sequences associated with specific pathogenic bacteria. The E. coli O157:H7 CHAS picks up definitive gene sequences associated with this […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method enumerates Staphylococcus aureus bacteria in foods and environmental samples. Tolerance guidelines are specific to the product tested. Petrifilm plates are a ready-to-use product developed by the 3M Company. Media are coated onto films, so that traditional media preparation is unnecessary, and both labour […]

GIFSQ — Global Institute for Food Safety & Quality Principle The GeneQuence Salmonella Microwell assay is a DNA probe-based diagnostic in kit format for the detection of Salmonella spp. in select foods and environmental surface samples. Results are available in approximately 48 hours. Salmonella-specific oligonucleotide probes labelled with horseradish peroxidase (HRP) are hybridized to ribosomal […]

GIFSQ — Global Institute for Food Safety & Quality Principle A gene-based assay for the detection of Escherichia coli O157:H7 and toxigenic E. coli O157:NM (non-motile) in foods. After enrichment, target microorganisms are concentrated from the sample, then each concentrated sample is transferred to an amplification tube containing specific primers and proprietary probes directed against […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method detects Listeria spp. on environmental surfaces to assess compliance with Sections 4 and 7 of the Food and Drugs Act and other relevant federal regulations. Positive results must be confirmed with a cultural method. The method has been validated for a variety of […]

GIFSQ — Global Institute for Food Safety & Quality Note: The method’s title block prints “Genequence”; the Principle section prints “GeneQuence”. Both are carried as printed. Principle This qualitative test detects Listeria monocytogenes in foods by DNA hybridization. Specific DNA probes for L. monocytogenes, labeled directly with horseradish peroxidase, give a colorimetric endpoint after broth-culture […]

GIFSQ — Global Institute for Food Safety & Quality Application This method applies to the rapid detection of Listeria spp. for checking compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act. It has been validated for raw meat, raw unprocessed seafood, raw frozen vegetables, cheese made from unpasteurized milk, […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method uses the ADIAFOOD Rapid Pathogen Detection System to identify Escherichia coli O157:H7 (E. coli O157:H7) strains and verotoxin-producing Escherichia coli O157:NM (E. coli O157:NM) strains from raw ground beef. After the sample enrichment procedure, the enrichment broth is subjected to a real-time polymerase […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is for the rapid identification of Yersinia enterocolitica colonies isolated by standard culture for Y. enterocolitica. It characterizes and identifies presumptive positive colonies isolated on validated plating media routinely used in the isolation of this pathogen, and can be used to determine compliance […]

GIFSQ — Global Institute for Food Safety & Quality (The source prints a registered mark after “Neogen” in the title, the Principle, and several materials; the mark does not appear in the kit codes. The mark is dropped here per GIFSQ practice.) Principle The Neogen Molecular Detection Assays use isothermal amplification of nucleic acid sequences […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is applicable to the detection of E. coli O157:H7/NM to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. The method has been validated using the BAX System Q7 instrument for […]

GIFSQ — Global Institute for Food Safety & Quality Principle The method is applicable to the enumeration of viable Escherichia coli to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. It has been validated for use with raw milk cheeses, raw meat, […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method detects Listeria species in a variety of environmental surface samples to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. GENE-UP Listeria spp. 2 (LIS 2) is a real-time polymerase chain […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is applicable to the rapid detection of Listeria monocytogenes to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. This method has been validated for use in the food categories raw […]

GIFSQ — Global Institute for Food Safety & Quality (Health Canada, Compendium of Analytical Methods, Volume 3 — Laboratory Procedure MFLP-110, August 2020.) Principle The method applies to the rapid detection of Listeria monocytogenes to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal […]

GIFSQ — Global Institute for Food Safety & Quality Application This method applies to the enumeration of viable Listeria species in environmental samples It cannot be used as a screening method for the absence of L. monocytogenes and other Listeria species; to determine absence, a qualitative (presence/absence) method must be used, e.g. MFHPB-30 This revised […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is applicable to the enumeration of coagulase-positive staphylococci (Staphylococcus aureus) to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. This method has been validated for use in all foods excluding […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is applicable to the enumeration of yeasts and molds to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. This method has been validated for use in all foods excluding strongly […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method enumerates Escherichia coli in foods to determine compliance with Sections 4 and 7 of the Food and Drugs Act and other relevant federal regulations. It is validated for all foods except strongly coloured foods (the primary dilution may need a higher dilution for […]

GIFSQ — Global Institute for Food Safety & Quality Health Canada, Compendium of Analytical Methods, Volume 3 — Laboratory Procedure MFLP-106, October 2018. (This method carries no “replaces” line.) Principle This method is applicable to the enumeration of coliforms to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs […]

GIFSQ — Global Institute for Food Safety & Quality Health Canada, Compendium of Analytical Methods, Volume 3 — Laboratory Procedure MFLP-105, August 2018. Health Products and Food Branch. Principle This method enumerates viable aerobic mesophilic bacteria to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method is applicable to the detection of Listeria species (spp.) for determining compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. It is validated for use on a variety of environmental surface samples. […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method applies to the rapid identification of Vibrio parahaemolyticus colonies isolated by standard culture for V. parahaemolyticus. It is used to characterize and identify presumptive positive colonies isolated on validated plating medium used in the isolation of this pathogen. The method can also be […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method supports rapid detection of Listeria spp. to show compliance with Sections 4 and 7 of the Food and Drugs Act and other applicable federal regulations, and it has been validated for a variety of environmental surfaces. The 3M Molecular Detection Assays use isothermal […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method applies to the detection of Salmonella spp. to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. Positive results must be confirmed with a cultural method. It has been validated for […]

GIFSQ — Global Institute for Food Safety & Quality Principle Compact Dry TC is a ready-to-use, chromogenic plate for the total viable bacterial count, developed by HyServe GmbH & Co. KG (Uffing, Germany). Compact Dry combines the features of traditional plate media with the modern features of dehydrated film media. Compact Dry TC is a […]

GIFSQ — Global Institute for Food Safety & Quality Principle 3M Petrifilm plates are a ready-to-use product. The medium is coated onto films, so traditional media preparation is unnecessary, and both labour and time are saved. The 3M Petrifilm Enterobacteriaceae Count Plate (or 3M Petrifilm EB Plate) is a sample-ready culture medium containing selective agents, […]

GIFSQ — Global Institute for Food Safety & Quality Principle This method gives a rapid genetic (gene-based) detection of Listeria species in selected foods and on environmental surfaces, to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and other relevant federal regulations. It applies to heat-processed and […]

GIFSQ — Global Institute for Food Safety & Quality Application This method is applicable to the rapid detection of Listeria monocytogenes to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. Positive results must be confirmed with a cultural method. This method is […]

GIFSQ — Global Institute for Food Safety & Quality Application This method is applicable to the rapid detection of Escherichia coli O157:H7, including non-motile (NM) strains, to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. Positive results must be confirmed with a […]

GIFSQ — Global Institute for Food Safety & Quality Application This method is used for the rapid isolation and identification of viable Listeria monocytogenes in ready-to-eat (RTE) meats and poultry, dairy products, fruit and vegetable-based products, and the smoked fish food type of the fish and seafood products food category, to determine compliance with Sections […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle Petrifilm plates are a ready-to-use product developed by the 3M Company, St. Paul, MN. The media are coated onto films, so traditional media preparation is unnecessary, and savings in both labour and time are realized. Coliforms are enumerated on the Petrifilm Coliform Count (CC) […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle This method enumerates aerobic bacteria in food products and food ingredients; it may also be used for environmental sampling (laboratory procedure MFLP-41). It can be used to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle Ready-to-use Petrifilm plates from 3M Company (St. Paul, MN) replace traditional medium preparation and save labour and time. Yeasts and moulds are enumerated on the Petrifilm Yeast and Mould Count (YM) plates, which carry dry medium and a cold-water-soluble gelling agent. A 1 mL […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle This method enumerates coliforms in foods and food ingredients to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act. Where an Official Method is specified for certain products, that method is followed. The method is not applicable […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle This method detects viable Listeria species in food and environmental samples. A portion of the product (or an environmental sample such as a sponge or swab) is enriched first in a primary broth, then in a secondary enrichment broth, plated onto a specified agar […]

GIFSQ — Global Institute for Food Safety & Quality This method (HPB Method MFHPB-29, March 2012) is applicable to the rapid detection of Listeria spp. for determining compliance with Sections 4 and 7 of the Food and Drugs Act. It has been validated for use in environmental samples and all foods except ready-to-eat meat and […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle Staphylococcus aureus makes a thermostable nuclease (TNase) that survives the heating steps used in food processing, much like staphylococcal enterotoxins. Viable cells may die off during processing and storage, so counting S. aureus in a finished food is not always a reliable guide to […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle Escherichia coli biotype 1 is enumerated in foods and food ingredients to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act. The conventional Most Probable Number (MPN) procedure takes 8 to 12 days to complete; the Direct […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle This method applies to the detection of Salmonella spp. in all foods, for compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and/or other relevant federal regulations. Any presumptive-positive result is confirmed with a cultural method. This method […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle This procedure estimates the number of viable Clostridium perfringens per g or mL of food. A portion of the product is mixed with a selective sulfite cycloserine agar by the pour-plate technique and incubated anaerobically. Typical black colonies are counted as presumptive C. perfringens; […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle A culture medium carrying enough nutrients for growth of most yeasts and moulds, together with antibiotics that suppress most bacteria, is inoculated with a measured quantity of the product and incubated; colonies are counted after 5 days at 22–25 °C. The count, in colony-forming […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle Certain staphylococci produce the enterotoxins that cause staphylococcal food poisoning, and with few exceptions this ability is limited to coagulase-positive strains. Known quantities (or dilutions) of a food sample are plated on the selective medium Baird-Parker (BP) agar, which has been validated for a […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle This is Health Canada’s cultural reference method for finding viable Salmonella species in foods and environmental surface samples, used to judge compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act and other relevant federal regulations. A portion of […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle Coliforms, faecal coliforms and Escherichia coli are used as indicator organisms: their presence in foods processed for safety can point to inadequate processing, post-processing contamination or microbial growth, and the presence of faecal coliforms and E. coli can point to faecal contamination. These terms […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle The Aerobic Colony Count (ACC) estimates the number of viable aerobic bacteria per g or mL of a food. A portion of the food is mixed with a plate count agar medium and incubated under defined conditions of time and temperature; each viable aerobic […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle Foodborne botulism is confirmed in the laboratory when botulinal toxin and/or viable Clostridium botulinum is found in a suspect food or a clinical specimen, alongside the clinical syndrome. Toxin is detected by injecting serum or extracts from foods and clinical specimens into mice, watching […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle This is Health Canada’s standard cultural reference method for determining the presence of Escherichia coli O157:H7 and non-motile (NM) strains in foods and environmental surface samples. Some E. coli O157:H7 strains lose expression of the H7 flagellar antigen and become non-motile, yet still carry […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle This method determines the presence of viable Listeria monocytogenes and other Listeria species in the product. A portion of the product is enriched first in a pre-enrichment broth (Palcam), then in an enrichment broth (UVM 2), plated onto a specified selective agar medium plus […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle Defects that could compromise the hermetic seal of food containers are detected by a physical examination and by tearing down the double seams of metal cans. The method applies to all two- and three-piece metal cans with conventional double seams, and to glass containers […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle Micro-leaks in hermetically sealed containers are found by subjecting an emptied, cleaned and dried container to a known vacuum or pressure and watching for air bubbles where air escapes. Applying a special dye at the test site lets a leak be seen visually as […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle A direct-reading Bourdon-tube gauge fitted with a self-sealing port is used to puncture the container and take a direct reading of its internal vacuum or pressure. The gauge measures the vacuum or pressure inside the sealed container relative to the standard atmospheric pressure at […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle The pH of a food — a measure of its hydrogen ion concentration — is found by dipping a single combination pH electrode directly into the food, or into a sample of the food that has been blended to a slurry. Modern pH meters […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle Simple stained smears and wet mounts are prepared from canned foods or other hermetically sealed containers that are microbiologically suspect, then examined under the microscope and the results recorded. Morphological characteristics of the organisms present are ascertained to determine whether they have public health […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle The contents of hermetically sealed foods are sampled aseptically and inoculated into suitable media to determine whether any viable microorganisms are present. A portion of the product is mixed with specified media and incubated under defined conditions of time and temperature. Under those conditions […]

GIFSQ — Global Institute for Food Safety & Quality Principle This document gives the laboratory formulation of the agar in the Media Glossary that falls under sections X–Y. For the medium it lists the basal medium ingredients with their quantities and the complete-medium preparation steps, so that the medium can be prepared in the laboratory […]

GIFSQ — Global Institute for Food Safety & Quality Principle This document gives the laboratory formulations of the agars, broths and other reagents in the Media Glossary that begin with the letter W. For each medium it lists the basal medium ingredients with their quantities, any supplement, and the complete-medium preparation steps, so that the […]

GIFSQ — Global Institute for Food Safety & Quality Principle This document gives the laboratory formulations of the agars, broths and other reagents in the Media Glossary that begin with the letter V. For each medium it lists the basal medium ingredients with their quantities and the complete-medium preparation steps, so that the medium can […]

GIFSQ — Global Institute for Food Safety & Quality Principle This document gives the laboratory formulations of the agars, broths and other reagents in the Media Glossary that begin with the letter U. For each medium it lists the basal medium ingredients with their quantities, any supplement, and the complete-medium preparation steps, so that the […]

GIFSQ — Global Institute for Food Safety & Quality Principle This document gives the laboratory formulations of the agars, broths and other reagents in the Media Glossary that begin with the letter T. For each medium it lists the basal medium ingredients with their quantities, any supplement, and the complete-medium preparation steps, so that the […]

GIFSQ — Global Institute for Food Safety & Quality Principle This document gives the laboratory formulations of the agars, broths and other reagents in the Media Glossary that begin with the letter S. For each medium it lists the basal medium ingredients with their quantities, any supplement, and the complete-medium preparation steps, so that the […]

GIFSQ — Global Institute for Food Safety & Quality Principle This document gives the laboratory formulations of the agars, broths and other reagents in the Media Glossary that begin with the letter P. For each medium it lists the basal medium ingredients with their quantities, any supplement, and the complete-medium preparation steps, so that the […]

GIFSQ — Global Institute for Food Safety & Quality Principle This document gives the laboratory formulations of the agars, broths and other reagents in the Media Glossary that begin with the letter M. For each medium it lists the basal medium ingredients with their quantities, any supplement, and the complete-medium preparation steps, so that the […]

GIFSQ — Global Institute for Food Safety & Quality Principle This document gives the laboratory formulations of the agars, broths and other reagents in the Media Glossary that begin with the letter L. For each medium it lists the basal medium ingredients with their quantities, any supplement, and the complete-medium preparation steps, so that the […]

GIFSQ — Global Institute for Food Safety & Quality Principle This document gives the laboratory formulations of the reagents in the Media Glossary that begin with the letter I. For each reagent it lists the basal medium ingredients with their quantities and the complete-medium preparation steps, so that the reagent can be prepared in the […]

GIFSQ — Global Institute for Food Safety & Quality Principle This document gives the laboratory formulations of the agars, broths and other reagents in the Media Glossary that begin with the letter H. For each medium it lists the basal medium ingredients with their quantities, any supplement, and the complete-medium preparation steps, so that the […]

GIFSQ — Global Institute for Food Safety & Quality Principle This document gives the laboratory formulations of the agars, broths and other reagents in the Media Glossary that begin with the letter G. For each medium it lists the basal medium ingredients with their quantities and the complete-medium preparation steps, so that the medium can […]

GIFSQ — Global Institute for Food Safety & Quality Principle This document gives the laboratory formulation of Fraser Broth (FB), also designated Modified Fraser Broth (MFB), a selective enrichment broth used in the analytical methods of the compendium. It lists the basal medium ingredients with their quantities, the ferric ammonium citrate supplement, and the complete-medium […]

GIFSQ — Global Institute for Food Safety & Quality Principle This document gives the laboratory formulations of the agars, broths and other reagents in the Media Glossary that begin with the letter E. For each item it lists the basal medium ingredients with their quantities, any supplement, and the complete-medium preparation steps, so that the […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle This SOP covers five standard preparations taken from Section D of the Media Glossary: three selective agar media for yeasts and moulds, one basal medium for amino-acid decarboxylase tests, and one chlorine disinfecting solution for containers. Each entry gives the ingredient quantities for the […]

GIFSQ — Global Institute for Food Safety & Quality Principle This section gives the compositions and the complete preparation instructions for the agars, broths, transport medium, plasma reagent and staining solution listed in the glossary: Campylobacter agar with charcoal and deoxycholate (CCDA), carbohydrate fermentation agar and broth for Listeria identification, Cary and Blair transport medium, […]

GIFSQ — Global Institute for Food Safety & Quality 1. Purpose This glossary lists the agars, broths, and other reagents prepared for use in food microbiological methods, from Baird-Parker medium to Buffered Peptone Water. Each entry gives the basal composition (quantities per 1.0 L of water unless stated) and the steps to prepare the complete […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle Cells of Listeria monocytogenes recovered from an environmental surface with a sponge or a swab are distributed across the wells of a 96-well plate containing enrichment broth. After incubation, a drop from each well is placed on differential agar in the same well pattern; […]

GIFSQ — Global Institute for Food Safety & Quality Principle This document gives the laboratory formulations of the agars and reagents in the Media Glossary that begin with the letters J and K. For each medium it lists the basal medium ingredients with their quantities, any supplement, and the complete-medium preparation steps, so that the […]

GIFSQ — Global Institute for Food Safety & Quality Principle This document gives the laboratory formulations of the agars, broths and other reagents in the Media Glossary that begin with the letter A. For each medium it lists the basal medium ingredients with their quantities, any supplement, and the complete-medium preparation steps, so that the […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle This is a supplement to all methods that detect Salmonella in food, food ingredients and environmental samples, to be used together with those methods. It provides additional information: sources of materials, critical steps, confirmation steps and helpful hints. For safety and environmental reasons, selenite […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle Results from a food microbiology laboratory are only as good as the sample and the way it is handled before analysis. This procedure lays out the good laboratory practices that apply to all compendium methods: how to store samples on receipt, how to prepare […]

GIFSQ — Global Institute for Food Safety & Quality Principle The most probable number (MPN) estimates the number of bacteria (coliforms in the coliform test) per unit of test material from the pattern of gas-positive tubes across a three-dilution series of five tubes each. The number of positive tubes in each of the three dilutions […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle The grid-lines of a hydrophobic grid-membrane filter (HGMF) are a hydrophobic print that divides the membrane into discrete growth compartments (grid-cells). The HGMF is treated as a multiple-tube Most Probable Number (MPN) procedure. The filter is inoculated by passing a sample suspension through the […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle Reliable microbiological results start before analysis: the sample taken from the lot must represent the lot, and it must reach the laboratory in the same state the consumer met it. This procedure covers how to collect sample units from a lot and how to […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle The limit of detection (LOD) of an indirect qualitative method is found by artificially inoculating food matrices with known target-bacterium concentrations over a range centred near the expected LOD, with several replicates at each spiking level. After cell equilibration, the alternative (candidate) method is […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle In method validation, method verification and recovery studies, artificially contaminated samples should contain the target organism in the same injured state that the food’s manufacturing process or natural conditions would produce. Injured target reference organisms are therefore prepared by exposing the culture to sub-lethal […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle A measured quantity of milk is filtered through a standard filter disc with a standard pump (the tester), and the character and amount of the residue retained on the disc are determined. The “off-the-bottom” method is designed for sampling milk from individual farmers’ milk […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle A small, standardised portion of the product is spread in a Howard mould counting chamber and examined under the microscope. At least 25 fields are scanned per mount at 90–125× with a field diameter of 1.382 mm, and each field is scored positive or […]

GIFSQ — Global Institute for Food Safety & Quality 1. Principle Alkaline phosphatase is destroyed by proper pasteurization, so its residual activity serves as an index of whether dairy products were adequately pasteurized. The enzyme liberates phenol from disodium phenylphosphate during incubation; the liberated phenol is coupled with 2,6-dichloroquinonechloroimide (CQC) to form a blue colour, […]