SOP: Detection of Argemone Seeds in Mustard

GIFSQ — Global Institute for Food Safety & Quality

Procedure

Weigh 50 g of powdered sample sufficient to yield 5–10 g nonvolatile ether extract

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Extract with 125 mL ethyl ether in a closed Erlenmeyer flask for 24 hrs with occasional shaking

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Filter the contents through Whatman filter paper No. 1 and wash the residue with two 50 mL portions of ethyl ether

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Evaporate the combined ether extract and dry the residual oil at 100°C

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Test the extracted oil for argemone oil as per the procedure in the manual on oils and fats

References

FSSAI (2016), Manual of method of analysis of foods — spices and condiments; Method no 19.0.