Ion exchange chromatography

A chromatography technique separating dissolved analytes on ion exchange resin beads: the exchangers release incoming sample ions as the eluting liquid’s pH, concentration, or ionic strength varies, usually with a gradient. Whether separation works by anion or cation exchange depends on the particular resin used. It purifies proteins, isolates charged flavour compounds, and analyzes ionic food components routinely — a proven workhorse method in both research and quality-control laboratories. Careful gradient elution sharpens the separation of closely related charged species.